2株耐盐碱根际促生细菌对盐碱胁迫下紫花苜蓿生长及生理的影响

Effects of two saline-tolerant rhizosphere growthpromotingbacteria on growth and physiology ofMedicago sativa under saline-alkali stress

摘要:

【目的】 研究接种耐盐碱根际促生细菌嗜麦芽寡养单胞菌JT4、普城沙雷氏菌JG1 对盐碱胁

迫条件下紫花苜蓿生长和生理特性的影响，为根际促生细菌JT4、JG1 在植物抗盐碱中的应用提供理论

依据。【方法】 采用室内盆栽试验，以紫花苜蓿为供试植物，以实验室前期筛选鉴定得到的耐盐碱及促

生能力均较好的根际促生细菌JT4、JG1 为供试菌株，设置盐碱胁迫及非盐碱胁迫处理，测定接种根际

促生细菌JT4、JG1 后紫花苜蓿幼苗生长和生理特性指标。【结果】 与对照相比，盐碱胁迫下接种JT4、

JG1 紫花苜蓿幼苗的株高分别提高了31. 64% 和43. 50%，根长分别提高了32. 78% 和7. 05%，鲜重分

别提高了100% 和60%，抗坏血酸过氧化物酶活性分别提高了27. 67% 和200. 58%，过氧化氢酶活性

分别提高了33. 33% 和22. 73%，可溶性蛋白含量分别提高了126. 94% 和118. 88%，叶绿素a 含量分别

提高了11. 12% 和16. 32%。【结论】 耐盐碱根际促生细菌JT4、JG1 能较好地缓解盐碱胁迫对紫花苜蓿

造成的损伤。

Abstract:

【Objective】 In this experiment，the effects of inoculation of halotolerant rhizosphere growth‐promotingbacteria，Stenotrophomonas maltophilia JT4 and Serratia plymuthica JG1，on plant growth and physiological characteristics of Medicago sativa under saline‐alkali stress were studied，so as to provide theoretical basis for the applicationof rhizosphere growth‐promoting bacteria JT4 and JG1 in saline‐alkali resistance of plants.【Method】 In indoorpot experiment，alfalfa was used as the test plant，and rhizosphere growth‐promoting bacteria JT4 and JG1 with goodsaline‐alkali tolerance and growth‐promoting ability were used as the test strains. The effects of inoculation of rhizospheregrowth ‐ promoting bacteria JT4 and JG1 on the growth and physiological characteristics of alfalfa seedlingswere studied under saline ‐alkali stress and non ‐saline stress.【Result】 Compared with the control，the plant height，root length，fresh weight，ascorbic acid peroxidase activity and catalase activity of alfalfa seedlings inoculated withJT4 and JG1 increased by 31. 64% and 43. 50%，32. 78% and 7. 05%，100% and 60%，27. 67% and 200. 58%，and33. 33% and 22. 73% respectively，soluble protein content increased by 126. 94% and 118. 88%，and chlorophyll acontent increased by 11. 12% and 16. 32%，respectively.【Conclusion】 Saline‐alkali tolerant rhizosphere growth‐promotingbacteria JT4 and JG1 can better alleviate the damage of alfalfa caused by saline‐alkali stress.