基于响应面法的非洲哈茨木霉AD-G4菌株固体发酵条件优化

Optimization of solid-state fermentation conditionsof Trichoderma afroharzianum AD-G4 strain usingresponse surface method

摘要:

【目的】 为了获得非洲哈茨木霉AD-G4 固体发酵的最佳条件。【方法】 利用单因素试验、BBD（Box-Behnken Design）响应面试验对非洲哈茨木霉AD-G4 菌株的固体发酵培养基、发酵条件等影响因素进行了优化。【结果】 适合此菌株产生的最佳培养基组成为：燕麦粉和小麦秸秆1∶1；最佳培养条件为初始pH 值7. 0，含水量52%，孢子浓度5. 40×106 个/mL，温度28 ℃ ，培养天数为8 d。【结论】 在优化的培养条件下，非洲哈茨木霉AD-G4 分生孢子产量达到1. 91×1010 个/g。通过培养条件优化确定了适合非洲哈茨木霉AD-G4 菌株产生分生孢子的条件，为高效开发非洲哈茨木霉生防菌剂奠定了基础。

Abstract:

【Objective】 To determine the optimal formulafor solid‐state fermentation of Trichoderma afroharzia-num AD ‐ G4.【Method】 This study employed single factor test and Box ‐ Behnken Design（BBD） response surfacemethodologyto optimize the solid fermentation medium and conditions for the T. afroharzianum AD ‐ G4 strain.【Result】 The findings indicated that the optimal medium composition consisted of a 1∶1 ratio of oat flour to wheatstraw. The ideal culture conditions were determined to be an initial pH of 7. 0，water content of 52%，spore concentrationof 5. 40×106 spores/mL，a temperature of 28 ℃，and a culture durationof 8 days.【Conclusion】 Under these cultureconditions，conidia yield of T. afroharzianum AD‐G4 reached 1. 91×1010 spores/g. The optimized culture conditionsprovide a foundation for the development of efficient T. afroharzianum biocontrol agent.