高原鼢鼠精子发生相关基因的季节性表达特征

Seasonal characteristics of spermatogenesis relatedgene expression in male plateau zokor

摘要:

【目的】 探明高原鼢鼠（Eospalax baileyi）精子发生相关基因表达的季节性特征。【方法】 分析高原鼢鼠繁殖期和非繁殖期睾丸形态、血清激素和睾丸特异性基因的季节特征。【结果】 高原鼢鼠在繁殖期睾丸重量增加，睾酮分泌升高，初级精母细胞、次级精母细胞、单倍体精细胞和精子中特异性基因的表达水平均上调。非繁殖期睾丸萎缩，睾酮分泌水平降低，初级精母细胞、次级精母细胞、单倍体精细胞和精子中特异性基因的表达水平下调。【结论】 高原鼢鼠睾丸形态、激素和基因表达具有季节性差异，非繁殖期高原鼢鼠睾丸精子发生停滞在精原细胞阶段。这为揭示高原鼢鼠睾丸发育机理提供了参考，对了解动物季节性繁殖的调控有重要意义。

Abstract:

【Objective】 Seasonal breeding animals exhibit annual periodic changes in gonadal morphology，physiologicalhormones levels and gene expression，which can improve reproductive efficiency and offspring survival rate.This study aims to explore the seasonal characteristics of spermatogenesis‐related gene expression in male plateau zokors.【Method】 We analyzed testicular morphology，serum hormones levels and testiculis ‐ specific genes betweenbreeding season and nonbreeding season in plateau zokors.【Result】 During the breeding season，male plateau zokortestes increased in size，testosterone levels were elevated，and a variety of germ cells were present in seminiferoustubles. The expression levels of specific genes were upregulated in primary spermatocytes，secondary spermatocytes，haploid spermatocytes and sperm. In contrast，during the nonbreeding season，the testes were small and the level oftestosterone were insufficient. The expression levels of specific genes were downregulated in primary spermatocytes，secondary spermatocytes，haploid spermatocytes and sperm.【Conclusion】 Our findings indicate seasonal differencesin testicular morphology，hormone and gene expression of plateau zokor. Spermatogenesis arrest at the spermatogoniastage during the nonbreeding season. These results provide a valuable insight for into the mechanisms of testicular activity，and understanding the regulation of seasonal reproduction of animals.