桃色欧文氏菌Cp2脂多糖对秀丽隐杆线虫生长发育的影响

Effect of Cp2 lipopolysaccharides of Erwinia per⁃sicina on the growth and development ofCaenorhabditis elegans

摘要:

【目的】 探究桃色欧文氏菌Cp2 脂多糖（Erwinia persicinaCp2 Lipopolysaccharides，Cp2-LPSe）对秀丽隐杆线虫（Caenorhabditis elegans）生长发育的影响。【方法】 以野生型秀丽隐杆线虫N2为材料，用试剂盒法提取纯化Cp2-LPSe，用不同浓度Cp2-LPSe（0、0. 157、0. 314、0. 471、0. 628 与0. 785 EU/mL）处理秀丽隐杆线虫，测定其咽泵次数、繁殖能力、体脂含量、寿命长短、运动速度、运动振幅、身体弯曲频率等指标。【结果】 各Cp2-LPSe 浓度的处理下，秀丽隐杆线虫的生长发育总体呈现先上升后下降的趋势，当浓度低于0. 314 EU/mL Cp2-LPSe 时，除体脂含量外各指标均显著高于对照（P＜0. 05），并在0. 314 EU/mL 达到最大值；浓度超过0. 314 EU/mL 时，各指标均显著低于对照（P＜0. 05）。【结论】 低于0. 314 EU/mLCp2-LPSe 处理秀丽隐杆线虫会提高其咽泵次数、运动速度、身体弯曲频率、运动振幅，在体脂含量不增高的前提下提高其繁殖能力、延长其寿命，促进其生长发育。本研究为Cp2-LPSe 作为一种延缓机体衰老制剂的开发提供理论依据。

Abstract:

【Objective】 The study is aimed to investigate the effect of Cp2 Lipopolysaccharides（ Cp2-LPSe） ofErwinia persicina on the growth and development of Caenorhabditis elegans.【Method】 In this experiment，Cp2-LPSe was extracted and purified by kit method using wild-type Caenorhabditis elegans N2 as the material. Differentconcentrations of Cp2-LPSe （0，0. 157，0. 314，0. 471，0. 628 and 0. 785 EU/mL） were used to treat Caenorhabditiselegans with different concentrations of Cp2-LPSe（ 0，0. 157，0. 314，0. 471，0. 628 and 0. 785 EU/mL） to determinethe number of throat pumps，reproductive capacity，body fat content，life span，movement speed，movement amplitude，body bending frequency and other indicators.【Result】 The experiment showed that the growth and developmentof Caenorhabditis elegans had a general trend of first rising and then decreasing under the treatment of eachCp2-LPSe concentration. When the concentration of Cp2-LPSe was 0. 314 EU/mL ，all indexes except body fatcontent reached a maximum and were significantly higher than those of the control （P＜0. 05），and when the concentrationexceeded 0. 314 EU/mL，all indexes were significantly lower than those of control （P＜0. 05）.【Conclusion】0. 314 EU/mL Cp2-LPSe treatment of Caenorhabditis elegans could increase the number of throat pumps，movementspeed，body bending frequency and movement amplitude，improve its reproductive ability，prolong its lifespan，and promote its growth and development without increasing its body fat content. This study provides a theoretical basisand practical basis for the development of Cp2-LPSe as a anti-aging agent.