不同盐溶液处理对3种野生芨芨草种子萌发特性的影响

Effects of different types of salt treatments on thegermination characteristics of three wild Ach⁃natherum splendens seeds

摘要:

【目的】 探讨芨芨草种子萌发对不同类型盐胁迫的响应。【方法】 以3 个不同地区（编号分别为YL、BT 和ZY）采集的芨芨草种子为材料，测定不同中性盐处理下其种子的萌发特性，利用隶属函数法对种子萌发期的耐盐性进行了综合评价，筛选萌发期耐盐性较强的种质资源。【结果】 低浓度NaCl对BT 芨芨草萌发具有促进作用，高浓度NaCl 对ZY、YL 和BT 芨芨草萌发具有抑制作用，Na₂SO₄ 对3种芨芨草的胚根生长均具有抑制作用。随着NaCl、Na₂SO₄ 和混合盐3 种盐溶液浓度的逐渐增加，3 种芨芨草种子的发芽率、发芽势、发芽指数、活力指数逐渐降低，胚芽长和胚根长逐渐减小。通过隶属函数法进行综合评价，3 种盐胁迫处理下芨芨草种子耐盐性强弱依次为：（ZY-混合盐）＞（BT-NaCl）＞（YL-混合盐）＞（YL-Na₂SO₄）＞（ZY-Na₂SO₄）＞（ZY- NaCl）＞（BT-混合盐）＞（BT- Na₂SO₄ ＞（YL-NaCl）。【结论】 ZY 和YL 芨芨草对混合盐耐受性最强，对NaCl 耐受性最差。BT 芨芨草对NaCl 耐受性最强，对Na₂SO₄耐受性最差

Abstract:

【Objective】 Achnatherum splendens seeds collected from three different regions were used as materialsto study the effects of different neutral salts on the germination characteristics of their seeds in order to explore theresponse of Achnatherum splendens seed germination to different types of salt stress.【Method】 The membershipfunction method was used to comprehensively evaluate the salt tolerance of Achnatherum splendens seeds at germinationstage in three different regions，and to screen out germplasm resources with stronger salt tolerance at germinationstage，in order to provide theoretical basis for Achnatherum splendens planting and new variety breeding in salinealkaliareas.【Result】 The results showed that low concentration of NaCl could promote the germination of BT splendens，high concentration of NaCl could inhibit the germination of ZY，YL and BT splendens，and Na₂SO₄ had inhibitoryeffects on the radicle growth of three splendens. With the increasing concentrations of NaCl，Na₂SO₄ and mixedsalts，the germination rate，germination potential，germination index and vigor index of the three Achnatherum splen-dens seeds gradually decreased，and the germ length and radicle length decreased gradually. According to the comprehensiveevaluation by membership function method，the salt tolerance of Splendens seeds under the three salt stresstreatments is as follows：（Zy-mixed salt） ＞ （BT-NaCl） ＞ （YL-mixed salt） ＞ （YL-Na₂SO₄） ＞ （ZYNa₂SO₄）＞ （ZY-NaCl） ＞ （BT-mixed salt） ＞ （BT-Na₂SO₄） ＞ （YL-NaCl）.【Conclusion】 ZY and YLAchnatherum splendens had the strongest tolerance to mixed salt and the worst tolerance to NaCl. BT splendens hadthe strongest tolerance to NaCl and the worst tolerance to Na₂SO₄.