接种不同根瘤菌株对紫花苜蓿生物量的影响

Effect of rhizobia inoculation on vegetative organ biomass of alfalfa

摘要:

【目的】 探究不同根瘤菌接种环境下紫花苜蓿幼苗各器官生物量变化和共生效应差异。 【方法】 以WL289HQ、WL319HQ、WL363HQ、陇中、清水、甘农9号紫花苜蓿（Medicago sativa）品种为材料，以接种不同根瘤菌菌株G3L3、G3L4、G3L7、LL7、LP3、G9L4、WLP2、S12531为处理，以不接菌处理为对照，分析接种根瘤菌后紫花苜蓿品种之间的差异、根瘤菌菌株之间的差异、品种和菌株的交互效应差异。【结果】 紫花苜蓿品种之间，WL298HQ的根、茎、叶生物量最高；根瘤菌菌株之间，G9L4的根、叶生物量最高，G3L7的茎生物量最高。品种和菌株的交互效应下，WL298HQ苜蓿接种菌株 G3L3、LL7、G9L4、LP3以及甘农9号苜蓿接种菌株G3L3、G3L7对叶、茎、根生物量的积累有促进效应；陇中苜蓿接种菌株G3L3、WLP2、LL7、LP3以及清水苜蓿接种菌株WLP2对叶、茎、根生物量的积累有抑制效应；WL298HQ 与菌株 LL7对茎、叶生物量的分配效应最强，匹配效果最好。 【结论】 WL298HQ和甘农9号接种菌株G3L3对叶、茎、根生物量的积累有促进效应，陇中和清水苜蓿接种菌株WLP2对叶、茎、根生物量的积累有抑制效应，紫花苜蓿与根瘤菌共生对生物量干重的分配效应遵循：茎＞叶＞根。

Abstract:

【Objective】 To explore the biomass changes and symbiotic effect differences in various organs of Alfalfa Seedlings under different rhizobial inoculation environments，【Method】 Medicago sativa （WL289HQ， WL319HQ，WL363HQ，Longzhong，Qingshui，Gannong 9） were used as materials. Rhizobia strains G3L3，G3L4， G3L7，LL7，LP3，G9L4，WLP2，S12531 were inoculated as treatments. Samples without rhizobia inoculation were- usedas control. The differences among M. sativa cultivars after inoculation with rhizobia，the differences among rhizobial strains，and the interactive effect differences between cultivars and strains were comparatively analyzed.【Result】 Among different alfalfa varieties，WL298HQ had the highest biomass of root，stem and leaf. Among different mycorrhizal treatments，the root and leaf biomass of G9L4 was the highest，while the stem biomass of G3L7 was the highest. Under the interactive effect of variety and strain，the WL298HQ alfalfa inoculated with strains G3L3，LL7，G9l4， and LP3 as well as the gannong 9 alfalfa inoculated with strains G3L3，G3L7 showed enhanced leaf，stem，and root biomass accumulation. Longzhong inoculated with strains G3L3，WLP2，LL7，and LP3 as well as the Qingshui inoculated strain WLP2 in longum showed inhibitory effects on the accumulation of leaf，shoot，and root biomass. WL298HQ showed the strongest partition effect with strain LL7 on the biomass of stem and leaf，with the best matching effect.【Conclusion】 The WL298HQ and Gannong 9 inoculated strain G3L3 showed positive effect on leaf，stem and root biomass accumulation. The inhibitory effect of WLP2，an inoculated strain with long neutralizing Medicago clear，on the accumulation of leaf，shoot and root biomass，and the partition effect of the symbiosis between M. sativa and rhizobia on biomass dry weight followed：stem＞leaf＞root.