10份藏沙蒿种质材料的耐寒性评价

Evaluation of cold tolerance of different Artemisia wellbyi germplasm

摘要:

【目的】 评价10份藏沙蒿（Artemisia wellbyi）种质的耐寒性差异。【方法】 以10份不同海拔生境的藏沙蒿种质为试验材料，在20 ℃（对照）、16 ℃、12 ℃和 8 ℃低温光照培养箱中进行萌芽试验，测定不同温度下的种子发芽势、发芽率、根长、芽长等指标，在20 ℃（对照）和4 ℃低温下测定苗期的逆境生理指标，采用主成分分析和隶属函数相结合的方法对10份藏沙蒿种质进行耐低温能力的综合评价。 【结果】 随着温度的降低，10份藏沙蒿的发芽率、发芽势、发芽指数、活力指数、胚根长、胚芽长均低于对照，并且种子的耐低温能力存在显著差异；在低温胁迫下超氧化物歧化酶（SOD）、过氧化氢酶（CAT） 活性和可溶性蛋白（SP）、可溶性糖（SS）含量均增加，脯氨酸（Pro）含量和过氧化物酶（POD）活性不同材料之间存在一定差异性（P＜0.05）。【结论】 采用隶属函数法对主成分筛选的7个指标的综合评价结果表明，2号藏沙蒿种质耐寒性较强，37号藏沙蒿种质材料耐寒性较弱。

Abstract:

【Objective】 In order to evaluate the cold resistance difference of 10 Artemisia wellbyi germplasm， 【Method】germination tests were carried out in low-temperature light incubators at 20 ℃ （control），16 ℃，12 ℃ and 8 ℃ using 10 A. wellbyi germplasm at different altitudes. The germination potential，germination rate，root length， bud length and other indexes were measured at different temperatures. The stress physiological indexes were measured at 20 ℃ （control） and 4 ℃，and the low temperature tolerance ability was comprehensively evaluated by combining principal component analysis and membership function.【Result】The results showed that with the decrease of temperature，the germination rate，germination potential，germination index，vigor index，radicle length and germ length of 10 A. wellbyi seeds were lower than those of the control，and there were significant differences in the low temperature tolerance of 10 A. wellbyi seeds. Under low temperature stress，the activities of superoxide dismutase （SOD） and catalase （CAT） and the contents of soluble protein （SP） and soluble sugar （SS） were increased，and the contents of proline （Pro） and peroxidase （POD） activity were different among different materials （P＜0. 05）. 【Conclusion】 The results of comprehensive evaluation of 7 indexes of principal component screening by membership function method showed that the cold tolerance of No. 2 A. wellbyi germplasm was strong，while that of No. 37 A. wellbyi germplasm was weak.