红豆草非结构性碳水化合物含量对低磷胁迫的响应

Response of non-structural carbohydrates content to low phosphorus stress of sainfoin

摘要:

【目的】 探究低磷胁迫对耐低磷和磷敏感红豆草非结构性碳水化合物及磷含量的变化规律和响应情况。【方法】 本试验采用营养液沙培法，以材料6、5、1、10295和甘肃红豆草（GS）为研究对象， 设置常磷（1.00 mmol/L KH2 PO4 ，NP）和低磷（0.01 mmol/L KH2 PO4 ，LP）胁迫处理，分别在处理0 d、 12 d和24 d进行采样，测定其地上部分和地下部分可溶性糖、淀粉、还原性糖、蔗糖和磷含量。【结果】 随着低磷胁迫程度的加剧，各材料地上部分的可溶性糖含量、淀粉含量和蔗糖含量均先降低后升高，还原性糖含量下降，而地下部分可溶性糖含量和还原性糖含量呈降低趋势，淀粉含量先增加后降低，蔗糖先降低后增加；随着胁迫天数的增加，磷含量呈现降低趋势。【结论】 材料5耐低磷能力较强，材料1耐低磷能力较弱。

关键词:红豆草;低磷胁迫;非结构性碳水化合物含量;隶属函数法

Abstract:

【Objective】 This study aimed to investigate the changes and responses of non-structural carbohydrate and phosphorus content in low-phosphorus-tolerant and phosphorus-sensitive sainfoin under low phosphorus stress.【Method】 This experiment adopted a nutrient solution sand culture method in which materials 6，5，1，10 295 and Onobrychis viciaefolia ‘Gansu’ were used as experimental materials. These materials were treated with constant normal phosphate （1. 00 mmol/L KH2 PO4 ，NP） and deficient phosphate stress （0. 01 mmol/L KH2 PO4 ，LP）. Samples were collected at 0 d，12 d and 24 d，respectively，and the contents of soluble sugar，starch，reducing sugar， sucrose and phosphorus in stem and leaf，root were determined.【Result】 Underlow-phosphorous stress，within the stems and leaves ，soluble sugar，starch，and sucrose content initially reduced and then increased，while the reducing sugar content consistently decreased. In the roots，soluble sugar content and reducing sugar content showed a trend of decrease，starch content initially increased and then decreased，and sucrose contentinitially decreased and then increased. Phosphorus content decreased with increasing duration of stress.【Conclusion】 Based on a comprehensive analysis using the membership function method，material 5 had a strong tolerance to low-phosphorus，and material 1 had a weak tolerance low-phosphorus.

Key words:sainfoin;low phosphorus stress;non-structural carbohydrate content;membership function method