10个燕麦品种对叶斑病抗性的鉴定与评价

Identification and evaluation of different Avena sativa varieties resistance to leaf spot

摘要:

【目的】 探究不同燕麦品种对叶斑病菌的生理响应及综合抗病能力。【方法】 测定了燕麦（Av- ena sativa）的 10个品种与抗病相关的生理指标，综合评价了各品种的抗病性。【结果】 接种燕麦德氏霉 （Drechslera avenae）后，牧王、爱沃、燕王和白燕7号等4个品种的发病率、病情指数分别低于19.05%、 8. 93，抗病性相对较强，而甜燕麦、青海444、陇燕3号、定引1号、定燕2号和牧乐思的发病率、病情指数分别高于21.43%、10.71，抗病性较弱；抗病性较强品种的叶绿素（Chl）、可溶性蛋白（SP）含量下降幅度较小，相对电导率（REC）、丙二醛（MDA）含量上升幅度较小，可溶性糖（SS）含量、过氧化物酶 （POD）、超氧化物歧化酶（SOD）、苯丙氨酸解氨酶（PAL）、多酚氧化酶（PPO）活性上升显著（P＜ 0. 05），抗病性较弱的品种与其相反。【结论】 10个燕麦品种分别为高抗（牧王、爱沃和燕王）、中抗（白燕 7号）、中感（甜燕麦、青海444、陇燕3号、定引1号）及感病（定燕2号和牧乐思）。

Abstract:

【Objective】 In order to explore the physiological response and comprehensive disease resistance of dif ferent oat to leaf spot.【Method】 The physiological indexes related to disease resistance of 10 A. sativa varieties weredetermined and the disease resistance of each variety was comprehensively evaluated.【Result】 The results showed that the disease incidence and disease index of Muwang，Aiwo，Yanwang and Baiyan 7 after inoculation with Drechslera avenae were lower than 19. 05% and 8. 93，respectively，and disease index of the four varieties were rela tively better，while the disease incidence and disease index of sweet oat，Qinghai 444，Longyan 3，Dingyin 1，Dingyan 2 and Mulesi were higher than 21. 43% and 10. 71，respectively，and the disease resistance was relatively inferiorer. The descent range of chlorophyll （Chl） and soluble protein （SP） content in resistance varieties were comparatively less；The rising range of relative electrical conductivity （REC） and malondialdehyde （MDA） content in resistance va rieties was comparatively less；Moreover，soluble sugar （SS） content，peroxidase （POD），superoxide dismutase （SOD），phenylalanine ammonia lyase （PAL），polyphenol oxidase （PPO） activity increased significantly in resistant varieties （P＜0. 05），conversely，the results in inferiorer disease resistance varieties was opposite.【Conclusion】 The comprehensive evaluation D value obtained by the membership function method can divide the 10 oat varieties into high resistance （Muwang，Aiwo and Yanwang），medium resistance （Baiyan 7），moderately susceptible （sweet oat， Qinghai 444，Longyan 3 and Dingyin 1） and susceptible （Dingyan 2 and Mulesi）