深绿木霉T2处理土壤对紫花苜蓿光合作用的影响

Effects of Trichoderma atroviride T2 treatment on photosynthesis of alfalfa

摘要:

【目的】 探究深绿木霉T2（Trichoderma atroviride T2）处理土壤后对紫花苜蓿光合作用的影响。【方法】 采用浓度为1×107个/g的T2孢子悬浮液处理紫花苜蓿盆栽幼苗的土壤，在土壤湿度、环境温度分别为70%、30 ℃条件下测定其对紫花苜蓿幼苗叶绿素含量与光合参数的影响。【结果】 T2处理土壤对紫花苜蓿的叶绿素含量及光合速率均有提高作用，且效果明显，与对照相比，当T2处理土壤第 7 天时，处理组紫花苜蓿幼苗叶绿素含量、光合参数分别与对照相比差异最大，其中叶绿素a、叶绿素b、 总叶绿素含量、净光合速率和水分利用率较对照分别提高了86.96%、81.58%、89.66%、104.06%和 174. 05%，类胡萝卜素、气孔导度和蒸腾速率分别比对照降低了44.74%、32.53%和33.93%。【结论】 深绿木霉T2土壤处理可促进紫花苜蓿的生长发育。

Abstract:

【Objective】 To investigate the effect of Trichoderma atroviride T2 treatment on the photosynthesis of alfalfa.【Method】 The soil of alfalfa potted seedlings was treated with Trichoderma atroviride T2 spore suspen sion at a concentration of 1×107 spores/g. The effects of T2 spore suspension on chlorophyll content and photosyn thetic parameters of alfalfa seedlings were measured under conditions of 70% soil moisture and an ambient tempera ture of 30 ℃，respectively.【Result】 Soil treatment with T2 significantly enhanced both chlorophyll content and pho tosynthetic rate in alfalfa seedlings. Compared with the control，the chlorophyll content and photosynthetic parameters of alfalfa seedlings in the treated group had the greatest difference on the 7th day of T2 soil treatment. Specifically， the contents of chlorophyll a，chlorophyll b，total chlorophyll，net photosynthetic rate and water utilization rate were in creased by 86. 96%，81. 58%，89. 66%，104. 06% and 174. 05%，respectively. In contrast，carotenoids，stomatal con ductivity，and transpiration rate decreased by 44. 74%，32. 53% and 33. 93%，respectively.【Conclusion】 Soil treat ment with T. atroviride T2 significantly promotes the photosynthetic efficiency and water use of alfalfa，thereby en hancing its growth and development.