2,3-丁二醇诱导草地早熟禾抗褐斑病的生理响应研究

Physiological response of anti-brown patch induction in kentucky bluegrass with 2,3-butanediol

摘要:

【目的】 探究2，3‐丁二醇（2，3‐BD）施用对草地早熟禾的诱导抗病效果。【方法】 以草地早熟禾品种公园作为试验材料，2，3‐BD为诱导剂，立枯丝核菌为接种病原菌。设置不诱导接菌（w）、诱导接菌（2，3‐BD+w）、诱导不接菌（2，3‐BD）处理。新型诱导剂2，3‐BD进行根部诱导并以草地早熟禾褐斑病菌撒播接菌处理，研究其诱发草地早熟禾的防御反应机制。【结果】 2，3‐BD诱导提高苯丙烷代谢途径中酶的活性，并改变氧化还原相关酶参与2，3‐BD诱导草地早熟禾抗病性的过程。苯丙氨酸解氨酶 （PAL）活性第1天达到最大值，且与对照差异显著。查尔酮异构酶（CHI）活性各时期均高于对照。4 香豆辅酶A连接酶（4CL）活性2次升至最高且与对照差异显著（P＜0.05）。超氧化物歧化酶（SOD）活性前期高于对照，第7天受到抑制低于对照，过氧化氢酶（CAT）活性第1天受抑制，之后明显高于对照，过氧化物酶（POD）活性也相应提高。2，3‐BD诱导下草地早熟禾体内次生代谢物含量增加，总酚， 黄酮和木质素作为抗菌物质参与了草地早熟禾抗病过程。诱导处理草地早熟禾总酚含量在第4天达到最大，为1.12 mg/g，对照为0.94 mg/g。黄酮含量第8天与病原菌侵染相比增加了0.37 mg/g。木质素含量在第8天为0.601 D280 /g，第10天为0.625 D280 /g。【结论】 适宜浓度2，3‐BD诱导能够引发草地早熟禾抗病相关酶活性变化，产生抗菌物质引起植物抗病生理响应，抵御病原物的侵染。

Abstract:

【Objective】 The purpose of this experiment was to explore the induced disease resistance effect of 2， 3‐butanediol（2，3‐BD） application on Kentucky bluegrass.【Method】 Kentucky bluegrass （park） was used as the test material，2，3‐BD was used as the inducer，and Rhizoctonia regussiana was used as the inoculator to set up the induced non‐inoculant bacteria （w），induced non-inoculant bacteria （2，3‐BD+w） and induced non-inoculant bacteria （2，3‐BD）. A new type of inducer，2，3‐BD was used to induce roots and inoculated with Kentucky bluegrass brown patch by seeding method， and the mechanism of defense response induced by the new inducer was discussed.【Result】 The results showed that 2，3‐BD induced increased the enzyme activity in the metabolic pathway of phenylpropane，and changed the activity of REDOX related enzymes involved in the improvement of disease resistance of Kentucky blue grass induced by 2，3-BD. The activity of phenylalanine aminolyase （PAL） reached the maximum on the 1th day， and the difference was significant compared with the contrast. The activity of chalcone isomerase （CHI） was higher than that of the contrast. 4 Coumadin coA ligase （4CL） activity increased to the highest twice and the difference was significant compared with the contrast. The activity of superoxide dismutase （SOD） in the early stage was higher than that of the control group，and the activity of catalase （CAT） was lower than that of the control group，and the ac tivity of peroxidase （POD） was higher than that of the control group. These results indicated that REDOX enzymes Participate in the early Kentucky bluegrass internal were involved in the improvement of disease resistance induced by 2，3‐BD through dynamic balance with active oxygen species in Kentucky bluegrass. The content of secondary me tabolites in Kentucky bluegrass increased under 2，3‐BD induction. Total phenols，flavonoids and lignin participated in the disease resistance process of Kentucky bluegrass as antibacterial substances. The maximum content of total phe nol in induction treatment was 1. 12 mg/g on the 4th day，while that in control was 0. 94 mg/g. Flavonoid content in creased by 0. 37 mg/g compared with that of pathogen infection on day 8. The lignin content was 0. 601 D280 /g on the 8th day and 0. 625 D280 /g on the 10th day.【Conclusion】It was concluded that induction of appropriate concentration of 2，3‐BD could induce changes in enzyme activity related to disease resistance of Kentucky bluegrass and produce antibacterial substances to induce physiological response to disease resistance and resist infection of pathogen.