不同植物激素对红豆草种子萌发的影响

Effect of different phytohormones on seed germination of Onobrychis viciifolia

摘要:

【目的】探究4种外源植物激素对红豆草(Onobrychis viciifolia)种子萌发的影响，解决种植过程中存在的发芽缓慢，发芽率低和出苗不整齐的问题。【方法】以甘肃红豆草种子为试验材料，研究三十烷醇(TA)、6-苄基腺嘌呤(6-BA)、油菜素内酯(BR)和复硝酚钠(CSN)在不同浓度下对红豆草种子发芽率、发芽势、发芽指数、胚根和胚芽长、贮藏物质和抗氧化酶活性的影响。【结果】不同浓度的TA、6-BA、BR和CSN 4种激素对红豆草种子的萌发都会产生影响，其中0.4 mg/L TA，10 mg/L 6-BA，0.08 mg/L BR和2 mg/L CSN对种子萌发的促进效果最显著，发芽率较对照分别提高了13.1%、24.7%、47.8%、27.5%，也增加了种子萌发过程中贮藏物质的含量和抗氧化酶活性；而浓度过高则会对红豆草种子的萌发产生抑制效果。【结论】4种外源激素对红豆草种子萌发均具有不同程度的调控作用，在红豆草种子萌发时，添加适量的植物激素对调控红豆草种子的质量和生长具有极其重要的作用。

Abstract:

【Objective】To examine the effects of four exogenous phytohormones on sainfoin seed germination and to address the problems of slow germination, low germination rate and irregular emergence during planting. 【Method】Using sainfoin seeds as the experimental materials, we investigated the effects of different concentrations of tricosanol (TA), 6-benzyladenine (6-BA), oleuropein lactone (BR), and sodium cisnitrophenate (CSN) on germination rate, germination potential, germination index, radicle and plumule length, storage material, and antioxidant enzyme activities. 【Result】All four hormones affected sainfoin seed germination across the concentrations tested. The most effective treatments were 0.4 mg/L TA, 10 mg/L 6-BA, 0.08 mg/L BR, and 2 mg/L CSN, which increased the germination rate by 13.1%, 24.7%, 47.8%, and 27.5%, respectively, compared with the control. These treatments also increased storage-substance content and antioxidant enzyme activity during germination, whereas excessively high concentrations inhibited germination. 【Conclusion】The four exogenous hormones regulated sainfoin seed germination to differing, indicating that applying an appropriate amount of phytohormone plays an important role in promoting germination. This study provides a theoretical reference for seedling practical production and application.